Hľadali ste: Thermo Fisher Scientific

Dodávateľ: Thermo Fisher Scientific

Opis: DreamTaq™ DNA Polymerase is an enhanced <i>Taq</i> DNA polymerase optimised for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional <i>Taq</i> DNA polymerase. DreamTaq™ DNA Polymerase uses the same reaction set-up and cycling conditions as conventional <i>Taq</i> DNA polymerase. Extensive optimisation of reaction conditions is not required. The enzyme is inhibited by dUTP but can incorporate modified nucleotides. Applications include routine PCR amplification of DNA fragments up to 6 kb from genomic DNA and up to 20 kb from viral DNA, RT-PCR, and generation of PCR products for TA cloning.

Dodávateľ: Thermo Fisher Scientific

Opis: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant <i>Taq</i> DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.

Katalógové číslo: (ER1821)

Dodávateľ: Thermo Fisher Scientific

Opis: NGGCC sites and cuts best at 50 °C in G buffer.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (ER0421)

Dodávateľ: Thermo Fisher Scientific

Opis: CCT sites and cuts best at 37 °C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (R0441)

Dodávateľ: Thermo Fisher Scientific

Opis: ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. This nucleotide is used in different molecular biology applications, and it has greater than 99% purity.

Merná jednotka: 1 * 1 KIT

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Dodávateľ: Thermo Fisher Scientific

Opis: GeneJET™ Plasmid Maxiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. GeneJET™ Plasmid Maxiprep Kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Dodávateľ: Thermo Fisher Scientific

Opis: DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced <i>Taq</i> polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Dodávateľ: Thermo Fisher Scientific

Opis: DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl₂ and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Certifikáty

Katalógové číslo: (FD1014)

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Merná jednotka: 1 * 1 KIT

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Dodávateľ: Thermo Fisher Scientific

Opis: Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.

Dodávateľ: Thermo Fisher Scientific

Opis: The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Dodávateľ: Thermo Fisher Scientific

Opis: PCR Master Mix is a 2X concentrated solution of <i>Taq</i> DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Certifikáty

Katalógové číslo: (FD0644)

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Merná jednotka: 1 * 1 KIT

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Dodávateľ: Thermo Fisher Scientific

Opis: FastDigest™ XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest™ and FastDigest™ Green reaction buffers.

Dodávateľ: Thermo Fisher Scientific

Opis: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Katalógové číslo: (B49)

Dodávateľ: Thermo Fisher Scientific

Opis: Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Merná jednotka: 1 * 1 KIT

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