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Dodávateľ: Thermo Fisher Scientific
Opis: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

Dodávateľ: Thermo Fisher Scientific
Opis: O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.

Katalógové číslo: (FD1254)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (FD0663)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
Merná jednotka: 1 * 1 KIT


Dodávateľ: Thermo Fisher Scientific
Opis: FastDigest XbaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest green reaction buffers.

Dodávateľ: Thermo Fisher Scientific
Opis: The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
Katalógové číslo: (EP0282)
Dodávateľ: Thermo Fisher Scientific
Opis: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.
Merná jednotka: 1 * 1 KIT


Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Dodávateľ: Thermo Fisher Scientific
Opis: RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Dodávateľ: Thermo Fisher Scientific
Opis: The IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose

Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Katalógové číslo: (EN0771)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA, such as cDNA and primers are not cleaved. dsDNase is easily inactivated by moderate heat treatment (55 °C). These features make dsDNase an excellent choice for gDNA elimination prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.
Merná jednotka: 1 * 1 items


Katalógové číslo: (ER0593)
Dodávateľ: Thermo Fisher Scientific
Opis: GGCCGC sites and cuts best at 37 °C in O buffer.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (EP0111)
Dodávateľ: Thermo Fisher Scientific
Opis: T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.
Merná jednotka: 1 * 1 KIT


Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Katalógové číslo: (K1612)
Dodávateľ: Thermo Fisher Scientific
Opis: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.
Merná jednotka: 1 * 1 KIT


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