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Dodávateľ: Thermo Fisher Scientific
Opis: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Katalógové číslo: (FD0974)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (FD1254)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (FD0663)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
Merná jednotka: 1 * 1 KIT


Dodávateľ: Thermo Fisher Scientific
Opis: FastDigest XbaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest green reaction buffers.

Katalógové číslo: (EM0821)
Dodávateľ: Thermo Fisher Scientific
Opis: CpG methyltransferase enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context. Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37 °C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (EP0282)
Dodávateľ: Thermo Fisher Scientific
Opis: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (EP0111)
Dodávateľ: Thermo Fisher Scientific
Opis: T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (K0512)
Dodávateľ: Thermo Fisher Scientific
Opis: DNA purification kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (K1612)
Dodávateľ: Thermo Fisher Scientific
Opis: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.
Merná jednotka: 1 * 1 KIT


Dodávateľ: Thermo Fisher Scientific
Opis: GeneJET™ Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant <i>E. coli</i> cultures. The kit utilises silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,<i> in vitro</i> transcription, and transfection of robust cell lines.

Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Dodávateľ: Thermo Fisher Scientific
Opis: Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start <i>Taq</i> DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Katalógové číslo: (EN0771)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA, such as cDNA and primers are not cleaved. dsDNase is easily inactivated by moderate heat treatment (55 °C). These features make dsDNase an excellent choice for gDNA elimination prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.
Merná jednotka: 1 * 1 items


Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

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