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Dodávateľ: Quantabio
Opis: PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.
Dodávateľ: Quantabio
Opis: Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ <i>Taq</i> DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified <i>Taq</i> DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.
Katalógové číslo: (ENZOENZPRT1010500)
Dodávateľ: ENZO LIFE SCIENCES
Opis: Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (ENZOENZPRT1000500)
Dodávateľ: ENZO LIFE SCIENCES
Opis: Optimised standard PCR for enhanced speed, yield, and specificity.
Merná jednotka: 1 * 1 KIT


Dodávateľ: Thermo Fisher Scientific
Opis: Boc-Pro-OH 99+%
Katalógové číslo: (25-6400-80)
Dodávateľ: Cytiva
Opis: illustra™ TempliPhi large construct DNA amplification kit (TempliPhi LC kit) is used to prepare templates for BAC or fosmid DNA sequencing.
Merná jednotka: 1 * 1.000 Tests


Dodávateľ: Quantabio
Opis: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.
Dodávateľ: Quantabio
Opis: PerfeCTa® qPCR SuperMixes are 2X concentrated, ready to use reaction cocktails containing all components except primers, probe and template for qPCR.

Katalógové číslo: (29050.)
Dodávateľ: BIOTIUM
Opis: Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq Gold by having a faster activation time and better shelf-life.
Merná jednotka: 1 * 500 EU


Dodávateľ: OMEGA BIOTEK
Opis: Isolate total RNA from cells or soft tissues using spin column.
Dodávateľ: Quantabio
Opis: PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
Dodávateľ: Quantabio
Opis: qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.
Dodávateľ: Quantabio
Opis: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
Dodávateľ: Quantabio
Opis: This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.
Dodávateľ: ENZO LIFE SCIENCES
Opis: Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Dodávateľ: OMEGA BIOTEK
Opis: MicroElute® Clean Up system, designed for rapid DNA clean up.
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