Hľadali ste: End-point PCR Enzymes and Kits

Dodávateľ: Quantabio

Opis: AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

Katalógové číslo: (ENZOENZPRT1010500)

Dodávateľ: ENZO LIFE SCIENCES

Opis: Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.

Merná jednotka: 1 * 1 KIT

New Product Predaj

Katalógové číslo: (ENZOENZPRT1000500)

Dodávateľ: ENZO LIFE SCIENCES

Opis: Optimised standard PCR for enhanced speed, yield, and specificity.

Merná jednotka: 1 * 1 KIT

New Product Predaj

Dodávateľ: Quantabio

Opis: The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq™ Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Dodávateľ: FINNZYMES REAGENTS

Opis: Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Dodávateľ: FINNZYMES REAGENTS

Opis: Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Dodávateľ: FINNZYMES REAGENTS

Opis: Phusion® High-Fidelity PCR Master Mix with HF Buffer is a convenient 2X mix containing Phusion® DNA Polymerase, nucleotides and optimised HF buffer including MgCl₂. Only template and primers need to be added by the user.

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Katalógové číslo: (EP0282)

Dodávateľ: Thermo Fisher Scientific

Opis: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.

Merná jednotka: 1 * 1 KIT

Predaj

Dodávateľ: FINNZYMES REAGENTS

Opis: Phusion® Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion® Hot Start II High-Fidelity DNA Polymerase is resistant to main PCR inhibitors present in blood and retains its activity at blood concentrations of up to 40% in the PCR reaction.

Dodávateľ: FINNZYMES REAGENTS

Opis: Phire® Hot Start II DNA Polymerase is faster, extremely robust, and capable of amplifying long DNA fragments with high yields. Phire® Hot Start II DNA Polymerase incorporates a dsDNA-binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to <i>Taq</i> DNA polymerase. In addition, the Hot Start technology allows complete reactivation of the enzyme in “zero-time” at standard cycling temperatures. This combination of features makes the polymerase an ideal solution for routine and high throughput PCR applications. Phire® Hot Start II DNA Polymerase delivers superior performance in conventional thermal cyclers as well as in fast instruments, such as the Piko® Thermal Cycler.

Dodávateľ: FINNZYMES REAGENTS

Opis: Phire™ Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The master mix containing Phire™ Hot Start II DNA polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors, such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle).

Katalógové číslo: (EDVO625)

Dodávateľ: EDVOTEK

Opis: Each PCR Bead contains <i>Taq</i> DNA polymerase, <i>Taq</i> DNA polymerase buffer, dNTP mixture and MgCl₂. Pack contains sufficient for 25 reactions.

Merná jednotka: 1 * 1 items

Predaj

Dodávateľ: FINNZYMES REAGENTS

Opis: Phusion® High-Fidelity PCR Master Mix with GC Buffer is a convenient 2X mix containing Phusion® DNA polymerase, nucleotides and optimised GC buffer including MgCl₂. Only template and primers need to be added by the user.

Dodávateľ: Thermo Fisher Scientific

Opis: DreamTaq™ PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq™ buffer, MgCl₂ and dNTPs. This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. DreamTaq™ DNA polymerase is an enhanced <i>Taq</i> polymerase optimised for high throughput PCR applications. It ensures robust amplification of PCR products up to 6 kb from genomic DNA and up to 20 kb from viral DNA.