Hľadali ste: End-point PCR Enzymes and Kits

Dodávateľ: ENZO LIFE SCIENCES

Opis: Increased sensitivity standard PCR mix for a broader range of samples, with enhanced speed, yield, and specificity.

Dodávateľ: Quantabio

Opis: AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

Certifikáty

Dodávateľ: Quantabio

Opis: AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

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Dodávateľ: VWR Chemicals

Opis: VWR® <i>Taq</i> DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

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Katalógové číslo: (1B1409-100RXN)

Dodávateľ: VWR Chemicals

Opis: Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.

Merná jednotka: 1 * 1 KIT

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Dodávateľ: Quantabio

Opis: AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II <i>Taq</i> DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

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Dodávateľ: VWR Chemicals

Opis: VWR® Red <i>Taq</i> DNA Polymerase Master Mix, which also contains an inert red dye, can be directly loaded onto an agarose gel without addition of electrophoresis loading buffers.

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Dodávateľ: VWR Chemicals

Opis: VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from <i>Thermus aquaticus</i>, and has a molecular weight of approximately 94 kDa. VWR® <i>Taq</i> DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

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Dodávateľ: VWR Chemicals

Opis: VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

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Dodávateľ: VWR Chemicals

Opis: VWR® <i>Taq</i> Plus is an optimised format of <i>Taq</i> DNA polymerase master mix and, therefore, is a good alternative to <i>Taq</i> DNA polymerase and <i>Taq</i> DNA polymerase master mix.

Katalógové číslo: (732-3267)

Dodávateľ: VWR Peqlab

Opis: PCR products of up to 12 kb and up to 40 kb can be achieved with the high performance peqGOLD 'Mid Range' and 'Long Range' PCR Systems respectively, suitable for both genomic and episomal templates. Applicable in place of standard <i>Taq</i> DNA polymerase for PCR amplifications when longer fragments and larger amounts are desirable, including sequencing, mapping and λ-phage or cosmid studies.

Merná jednotka: 1 * 1 items

Certifikáty Predaj

Katalógové číslo: (PEQL01-1018)

Dodávateľ: VWR Peqlab

Opis: 10X PCR Buffer, incomplete

Merná jednotka: 1 * 500 mL

Certifikáty Predaj

Dodávateľ: Quantabio

Opis: The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

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Dodávateľ: Quantabio

Opis: Superior speed and inhibitor tolerance.

Certifikáty

Dodávateľ: Apollo Scientific

Opis: Recombinant Taq plus DNA polymerase produced in <i>E.coli </i>5 U/µl.

Dodávateľ: Quantabio

Opis: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

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