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Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Product Image-1B1301-KIT

VWR® Protein Precipitation Kit

Katalógové číslo: (1B1301-KIT)
Dodávateľ: VWR Chemicals
Opis: The Protein Precipitation Kit utilises a simple trichloroacetic acid (TCA) based method to precipitate proteins in complex mixtures. Only protein in the sample is pelleted, while interfering salts and surfactants remain in the supernatant. A gentle acetone wash removes TCA and co-precipitants allowing the end-user to recover the protein in a small volume of buffer.
Merná jednotka: 1 * 1 KIT
Image Unavailable-33021-1

Thermal shift protein stability kits, GloMelt™

Dodávateľ: BIOTIUM
Opis: Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

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Product Image-786-603

Protein assay kits, RED 660™

Dodávateľ: G BIOSCIENCES
Opis: RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

Product Image-786-573

Bicinchoninic acid (BCA) reducing agent compatible protein Assay

Dodávateľ: G BIOSCIENCES
Opis: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

Image Unavailable-TNB-1213-L150

Foxp3 / Transcription Factor Staining Buffer Kit, Tonbo Biosciences

Katalógové číslo: (TNB-1213-L150)
Dodávateľ: TONBO BIOSCIENCE
Opis: Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry
Merná jednotka: 1 * 150 mL
Image Unavailable-CAYM10005020-96

Protein carbonyl colorimetric assay kit

Katalógové číslo: (CAYM10005020-96)
Dodávateľ: CAYMAN
Opis: The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.
Merná jednotka: 1 * 96 Tests
Image Unavailable-ENZOENZ51029K100

eFluxx-ID® multidrug resistance assay kit

Dodávateľ: ENZO LIFE SCIENCES
Opis: The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

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Product Image-ROCKMB-061-1000

Conjugate stabilisers

Dodávateľ: ROCKLAND
Opis: Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.

Product Image-ENZOENZ51023KP050

PROTEOSTAT® protein aggregation assay

Katalógové číslo: (ENZOENZ51023KP050)
Dodávateľ: ENZO LIFE SCIENCES
Opis: PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.
Merná jednotka: 1 * 50 Tests

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Product Image-786-005

Protein quantitation assay kits, Non-Interfering™ (NI™) Protein Assay

Katalógové číslo: (786-005)
Dodávateľ: G BIOSCIENCES
Opis: A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.
Merná jednotka: 1 * 1 KIT
Image Unavailable-ENZOBMLUW1000A0001

SUMO-QAPTURE-T® kit for isolation and enrichment of SUMOylated proteins

Katalógové číslo: (ENZOBMLUW1000A0001)
Dodávateľ: ENZO LIFE SCIENCES
Opis: The only commercially available kit for isolation and enrichment of SUMOylated proteins.
Merná jednotka: 1 * 10 Tests

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Product Image-GENO786-890

Bicinchoninic acid (BCA) protein assay

Dodávateľ: G BIOSCIENCES
Opis: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

Product Image-786-572

Micro Bicinchoninic Acid (BCA) protein assay

Dodávateľ: G BIOSCIENCES
Opis: The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).

Image Unavailable-GENO786-1084

Compatible Lowry protein assay

Dodávateľ: G BIOSCIENCES
Opis: These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.

Product Image-CAYM700510-96

L-Lactate Assay Kit

Katalógové číslo: (CAYM700510-96)
Dodávateľ: CAYMAN
Opis: A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
Merná jednotka: 1 * 1 items
Product Image-CAYM700480-96

Glycogen Assay Kit

Katalógové číslo: (CAYM700480-96)
Dodávateľ: CAYMAN
Opis: A simple and sensitive tool for assaying glycogen from tissue.
Merná jednotka: 1 * 1 items
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