Hľadali ste: Thermo Fisher Scientific

Dodávateľ: Thermo Fisher Scientific

Opis: Maleimide Activated Horseradish Peroxidase is for preparation of HRP conjugates with proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

Katalógové číslo: (PIER80370)

Dodávateľ: Thermo Fisher Scientific

Opis: (3-aminopropyl)tremethoxysilan

Merná jednotka: 1 * 100 g

Predaj

Katalógové číslo: (PIER34012)

Dodávateľ: Thermo Fisher Scientific

Opis: Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.

Merná jednotka: 1 * 250 mL

Predaj

Dodávateľ: Thermo Fisher Scientific

Opis: Blocker™ Casein in PBS or TBS are ready-to-use, solutions of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

Certifikáty

Katalógové číslo: (PIER32490)

Dodávateľ: Thermo Fisher Scientific

Opis: Pierce™ Recombinant Peroxidase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.

Merná jednotka: 1 * 0,5 mg

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Predaj

Katalógové číslo: (PIER34040)

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

Merná jednotka: 1 * 1 g

Predaj

Dodávateľ: Thermo Fisher Scientific

Opis: Pierce™ Protein G UltraLink™ and Protein G Plus UltraLink™ resins are useful for purification of antibodies and immunoprecipitation (IP). Samples containing IgG are incubated with the resin-immobilised Protein G in a buffer that facilitates binding. After non-IgG and non-antigen components of the sample are washed from the resin, the bound IgG and antigen may be recovered by elution.

Katalógové číslo: (PIER22317)

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific Pierce Sulfo-SMPB is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

Merná jednotka: 1 * 50 mg

Predaj

Katalógové číslo: (PIER22311)

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 angstroms).

Merná jednotka: 1 * 50 mg

Predaj

Katalógové číslo: (PIER22416)

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

Merná jednotka: 1 * 50 mg

Predaj

Dodávateľ: Thermo Fisher Scientific

Opis: These spin columns provide a fast, easy to use format for purifying GST-fusion proteins from cellular lysates. The glutathione is immobilised through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification.

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS-SS-Biotin is our highest quality formulation of this amine-reactive, cleavable biotinylation reagent, specially characterized for applications where product integrity and risk minimization are paramount. Sulfo-NHS-SS-biotin is used for cleavable attachment of biotin to protein and cell surface amines.

Katalógové číslo: (PIER88279)

Dodávateľ: Thermo Fisher Scientific

Opis: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

Merná jednotka: 1 * 1 KIT

Predaj

Dodávateľ: Thermo Fisher Scientific

Opis: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Dodávateľ: Thermo Fisher Scientific

Opis: Pierce™ microcentrifuge tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.