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Opis: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Katalógové číslo: 786-275

Merná jednotka: 1 * 1 KIT

Dodávateľ: G BIOSCIENCES

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Opis: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.

Katalógové číslo: PIER90054

Merná jednotka: 1 * 50 µG

Dodávateľ: Thermo Fisher Scientific

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Predaj

Opis: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

Katalógové číslo: PIER90053

Merná jednotka: 1 * 2 µG

Dodávateľ: Thermo Fisher Scientific

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Predaj

Opis: Neuronal Protein Extraction Reagent (N-PER), sufficient reagent to extract protein from ~10 g of cells or tissue, Pierce

Katalógové číslo: PIER87793

Merná jednotka: 1 * 100 mL

Dodávateľ: Thermo Fisher Scientific

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Opis: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Katalógové číslo: 786-277

Merná jednotka: 1 * 1 KIT

Dodávateľ: G BIOSCIENCES

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Opis: The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.

Katalógové číslo: 786-182

Merná jednotka: 1 * 1 KIT

Dodávateľ: G BIOSCIENCES

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Opis: InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.

Katalógové číslo: 786-241A

Merná jednotka: 1 * 1 KIT

Dodávateľ: G BIOSCIENCES

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Opis: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).

Katalógové číslo: ENZOBMLKI1050300

Merná jednotka: 1 * 300 µl

Dodávateľ: ENZO LIFE SCIENCES

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Opis: Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.

Katalógové číslo: PIER77610

Merná jednotka: 1 * 1 SET

Dodávateľ: Thermo Fisher Scientific

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Opis: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Katalógové číslo: 786-214

Merná jednotka: 1 * 10 items

Dodávateľ: G BIOSCIENCES

Certifikáty Predaj

Opis: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.

Katalógové číslo: PIER90056

Merná jednotka: 1 * 100 µG

Dodávateľ: Thermo Fisher Scientific

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Opis: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.

Katalógové číslo: 786-599

Merná jednotka: 1 * 5 mL

Dodávateľ: G BIOSCIENCES

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Opis: Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

Katalógové číslo: 786-283

Merná jednotka: 1 * 5 mL

Dodávateľ: G BIOSCIENCES

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Opis: Protein A Sepharose™ is a suspension of beads conjugated to native Protein A derived from <i>Staphylococcus aureaus</i>. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2 and rabbit.

Katalógové číslo: ROCKPA50-00-0005

Merná jednotka: 1 * 5 mL

Dodávateľ: ROCKLAND

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Opis: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.

Katalógové číslo: PIER20308

Merná jednotka: 1 * 1 KIT

Dodávateľ: Thermo Fisher Scientific

Predaj

Opis: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Katalógové číslo: 786-127

Merná jednotka: 1 * 1 KIT

Dodávateľ: G BIOSCIENCES

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