Hľadali ste: BIOWEST

Katalógové číslo: (L0207-500)

Dodávateľ: BIOWEST

Opis: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Merná jednotka: 1 * 500 mL

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Katalógové číslo: (L0950-100)

Dodávateľ: BIOWEST

Opis: Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

Merná jednotka: 1 * 100 mL

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Dodávateľ: BIOWEST

Opis: Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

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Dodávateľ: BIOWEST

Opis: Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

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Katalógové číslo: (L0014-100)

Dodávateľ: BIOWEST

Opis: Glutamine penicillin streptamycin acts against gram-negative and gram-positive bacteria.

Merná jednotka: 1 * 100 mL

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Dodávateľ: BIOWEST

Opis: Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

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Dodávateľ: BIOWEST

Opis: These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells <i>in vitro</i>. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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Katalógové číslo: (X0557-100)

Dodávateľ: BIOWEST

Opis: Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Merná jednotka: 1 * 100 mL

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Dodávateľ: BIOWEST

Opis: In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

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Katalógové číslo: (L0630-100)

Dodávateľ: BIOWEST

Opis: Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.

Merná jednotka: 1 * 100 mL

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Dodávateľ: BIOWEST

Opis: L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

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Dodávateľ: BIOWEST

Opis: Dulbecco's Modified Eagle Medium (DMEM) is a modification of Basal Medium Eagle (BME) which contains approximately four times as much of the vitamins and amino acids, and two to four times as much glucose (depending on the version) as the original formulation.

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Dodávateľ: BIOWEST

Opis: Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

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Dodávateľ: BIOWEST

Opis: Penicillin-Streptomycin acts against Gram-negative and Gram-positive bacteria.

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Dodávateľ: BIOWEST

Opis: Lymphosep is a standardised, high quality medium designed for simple and rapid isolation of PBMCs from treated whole blood. Lymphosep, like Ficoll™, has the optimised density 1,077 g/ml for lymphocyte separation to guarantee best and reliable results for your preparations.

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Dodávateľ: BIOWEST

Opis: Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.

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