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Product Image-EDVO225

Experimentation kits, DNA fingerprinting using restriction enzymes

Katalógové číslo: (EDVO225)
Dodávateľ: EDVOTEK
Opis: This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.
Merná jednotka: 1 * 1 KIT

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com


Product Image-BE-312

Experimentation kits, expression of a recombinant protein

Katalógové číslo: (BE-312)
Dodávateľ: G BIOSCIENCES
Opis: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).
Merná jednotka: 1 * 1 KIT
Product Image-USBIP3340-53C

Anti-Pericentriolar Material 1 Protein Rabbit Polyclonal Antibody

Katalógové číslo: (USBIP3340-53C)
Dodávateľ: UNITED BIOCHEMICALS
Opis: Anti-Pericentriolar Material 1 Protein Rabbit Polyclonal Antibody
Merná jednotka: 1 * 200 µl
Product Image-EDVO333

Experimentation kits, PCR-based Alu-Human DNA typing

Katalógové číslo: (EDVO333)
Dodávateľ: EDVOTEK
Opis: Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.
Merná jednotka: 1 * 1 KIT
Product Image-EDVO153

Experimentation kits, determination of protein molecular weight (polyacrylamide-based)

Katalógové číslo: (EDVO153)
Dodávateľ: EDVOTEK
Opis: Using prestained LyphoProteins, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers.
Merná jednotka: 1 * 1 KIT

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com


Product Image-EDVO105-C

Kits: mapping of restriction sites on plasmid DNA, Ready-to-Load™

Dodávateľ: EDVOTEK
Opis: DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

Product Image-EDVO275

Experimentation kits, simulation of HIV detection by western blot (agarose-based)

Katalógové číslo: (EDVO275)
Dodávateľ: EDVOTEK
Opis: The second assay used to confirm a positive HIV ELISA result is the Western blot. Using this kit, students separate protein samples from hypothetical patients on agarose gels. The proteins are then transferred to a membrane and simulated HIV proteins are detected.
Merná jednotka: 1 * 1 items
Product Image-EDVO112

Kits: analysis of Eco RI cleavage patterns of lambda DNA, Ready-to-Load™

Dodávateľ: EDVOTEK
Opis: Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.

Product Image-ICNA092850145

Chicken embryo extract, MP Biomedicals

Katalógové číslo: (ICNA092850145)
Dodávateľ: MP BIOMEDICALS
Opis: Chicken embryo extract prepared from nine to 12 day old eggs.
Merná jednotka: 1 * 20 mL

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com


Product Image-EDVO114-C

Kits: DNA paternity test, Ready-to-Load™

Dodávateľ: EDVOTEK
Opis: This experiment introduces students to the use of DNA fingerprinting in a simulated paternity determination. A child's DNA fingerprint is compared with his parents.

Product Image-EDVO207

Experimentation kits, southern blot analysis

Katalógové číslo: (EDVO207)
Dodávateľ: EDVOTEK
Opis: This experiment introduces students to Southern blotting as a tool for DNA fingerprinting in a hypothetical paternity determination. DNA fragments are first separated by agarose gel electrophoresis, then transferred to a nylon membrane and finally visualised by staining.
Merná jednotka: 1 * 1 items
Product Image-EDVO274

Experimentation kits, in search of the 'Kissing Disease'

Katalógové číslo: (EDVO274)
Dodávateľ: EDVOTEK
Opis: Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.
Merná jednotka: 1 * 1 items

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com


Product Image-EDVO300

Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Katalógové číslo: (EDVO300)
Dodávateľ: EDVOTEK
Opis: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.
Merná jednotka: 1 * 1 KIT
Image Unavailable-EDVO715

Restriction enzymes, Dryzymes®

Dodávateľ: EDVOTEK
Opis: The three most frequently used restriction enzymes are Eco RI, Bam HI and Hind III. Each enzyme catalyses cleavage at the defined base sequence. All enzymes are lyophilised and contain 1500 units. One unit is defined as the amount of enzyme required to digest 1,0 µg of lambda DNA in 60 minutes at 37 °C in a total reaction mixture of 50 µl.

Product Image-EDVO222

Transformation with blue and green fluorescent proteins

Katalógové číslo: (EDVO222)
Dodávateľ: EDVOTEK
Opis: The Green Fluorescent Protein (GFP) from the jellyfish <i>Aequorea victoria</i> is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.
Merná jednotka: 1 * 1 items

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com


Product Image-BE-501

Experimentation kits, antigen-antibody interactions

Katalógové číslo: (BE-501)
Dodávateľ: G BIOSCIENCES
Opis: This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.
Merná jednotka: 1 * 1 KIT
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