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Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR®, Rapid Transfer buffer 10X

Katalógové číslo: (N789-4L)

Dodávateľ: VWR Chemicals

Opis: Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

Merná jednotka: 1 * 4 L

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nehodí sa Každý

VWR®RapidBlock™, Blocking solution, 10x

Katalógové číslo: (M325-100ML)

Dodávateľ: VWR Chemicals

Opis: RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.

Merná jednotka: 1 * 100 mL

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nehodí sa Každý

Tris-Borate-EDTA (TBE) buffer, 10X

Dodávateľ: PanReac AppliChem

Opis: 10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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Phosphate buffered saline (PBS), 10X

Katalógové číslo: (786-027)

Dodávateľ: G BIOSCIENCES

Opis: PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

Merná jednotka: 1 * 500 mL

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nehodí sa Každý

Mini flip-top vial with 9 ml buffered peptone water broth, BPPFV9BPW

Katalógové číslo: (MMMPBPPFV9BPW)

Dodávateľ: 3M FOOD SAFETY

Opis: The 3M™ Mini Flip-Top Vial with 9 ml Buffered Peptone Water Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

Merná jednotka: 1 * 100 items

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nehodí sa Každý

Phosphate Buffered Saline

Katalógové číslo: (OXOIBR0014G)

Dodávateľ: THERMO FISHER DIAGNOSTICS

Opis: Cell culture buffer, Phosphate buffered saline tablets

Merná jednotka: 1 * 100 items

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nehodí sa Každý

Hybridisation buffers

Dodávateľ: G BIOSCIENCES

Opis: The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Mini flip-top vial with 9 ml maximum recovery diluent, BPPFV9MRD

Katalógové číslo: (MMMPBPPFV9MRD)

Dodávateľ: 3M FOOD SAFETY

Opis: The 3M™ Mini Flip-Top Vial with 9 ml Maximum Recovery Diluent is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

Merná jednotka: 1 * 100 items

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