Tlačiť

Hľadali ste: Primers+and+Probes


6 208  results were found

Spresniť výsledky Zoradiť podľa
SearchResultCount:"6208"

Sort Results

Zobraziť zoznam Easy View (Nové)

Ohodnotiť tieto výsledky vyhľadávania

Product Image-BIOPFS-SP0047

Food safe kits for identification of pathogenic microorganisms

Dodávateľ: BIOPREMIER
Opis: Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

Image Unavailable-733-0634

ABsolute™ qPCR Capillary Mixes

Dodávateľ: THERMO ABGENE
Opis: ABsolute™ qPCR Capillary Mixes contain an optimised reaction buffer specifically designed to provide the highest possible sensitivity, specificity and consistent end-point values in real time instruments that use glass capillaries, such as the Roche LightCycler®. The 2X mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Image Unavailable-733-1398

PerfeCTa® qPCR FastMix™ reaction mixes

Dodávateľ: Quantabio
Opis: FastMixes include buffers and stabilisers optimised for maximum PCR efficiency, reproducibility, and sensitivity with TaqMan® and TaqMan® MGB probe chemistries. A key component of PerfeCTa® qPCR FastMixes is AccuFast™ <i>Taq</i> DNA polymerase that enables instant activation and rapid polymerisation kinetics. Upon heat activation (20 seconds at 95 °C), the antibodies irreversibly denature releasing fully active, unmodified <i>Taq</i> DNA polymerase. This promotes specific and efficient primer extension with the convenience of room temperature reaction assembly. FastMixes deliver maximum PCR efficiency, sensitivity, specificity, and a robust fluorescent signal with either fast or conventional cycling conditions.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

Product Image-K0223

Maxima™ SYBR green/ROX QPCR master mix (2X)

Dodávateľ: Thermo Fisher Scientific
Opis: The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start <i>Taq</i> DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start <i>Taq</i> DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Certifikáty

Image Unavailable-733-0385

ABsolute™ qPCR Low ROX Mixes

Dodávateľ: THERMO ABGENE
Opis: ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Product Image-PRSI79-340

Anti-MAP2K6 Rabbit Polyclonal Antibody

Katalógové číslo: (PRSI79-340)
Dodávateľ: PROSCI
Opis: MEK6 is a member of MAPKK protein kinase family. By using degenerate oligonucleotide primers from the conserved kinase domains of MKK3 and MKK4 two human cDNAs and 1 murine cDNA encoding closely related proteins of the MKK family were cloned. The two human clones appear to be different isoforms of the same gene generated by differential splicing: the shorter clone was designated MKK6, encodes a 278-amino acid protein, while the longer clone, designated MKK6b, encodes a 334-amino acid protein. MKK6 is about 80% identical to MKK3 and 40% identical to MKK4. 1.7-kb human MKK6 transcript is highly expressed in skeletal muscle, while an MKK6b-specific probe detected mRNA bands of 1.8, 2.4, and 4.5 kb that are enriched in heart, skeletal muscle, pancreas and liver. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAPK mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
Merná jednotka: 1 * 100 µG
Image Unavailable-ENZOADI9502340100

IHC tissue primer

Katalógové číslo: (ENZOADI9502340100)
Dodávateľ: ENZO LIFE SCIENCES
Opis: Immunohistochemistry tissue primer is an enzyme blocking reagent.
Merná jednotka: 1 * 100 mL
Product Image-733-2543

VWR®, Taq DNA polymerase master mix

Dodávateľ: VWR Chemicals
Opis: VWR® <i>Taq</i> DNA Polymerase Master Mix is a ready to use 1,1X or 2X reaction mix. Simply add primers, template and water to carry out primer extensions and other molecular biology applications.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

Product Image-OXNTEXP-PCA001

PCR expansion

Katalógové číslo: (OXNTEXP-PCA001)
Dodávateľ: Oxford Nanopore Technologies
Opis: PCR Primers (PRM) and PCR Adapters (PCA).
Merná jednotka: 1 * 1 KIT
Product Image-733-2479

VWR®, Taq DNA polymerase, 200000 U

Katalógové číslo: (733-2479)
Dodávateľ: VWR Chemicals
Opis: Taq DNA polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kDa.
Merná jednotka: 1 * 1 KIT
Product Image-ENZOENZ450060001

Methylated DNA standard (mouse), universal

Katalógové číslo: (ENZOENZ450060001)
Dodávateľ: ENZO LIFE SCIENCES
Opis: Universal methylated DNA standard (mouse) with the included primers can be used in conjunction with Enzo blood and tissue methylation kit or the express methylation kits.
Merná jednotka: 1 * 20 Tests
Product Image-N224-500U

VWR®, Taq DNA polymerase, 500 U

Katalógové číslo: (N224-500U)
Dodávateľ: VWR Chemicals
Opis: <i>Taq</i> DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of <i> Taq</i> DNA Polymerase to a 25 µl PCR reaction.
Merná jednotka: 1 * 500 items

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com


Image Unavailable-APOSBIA1197-250G

Agaróza molecular sieve grade

Dodávateľ: Apollo Scientific
Opis: Agarose Molecular Screening 8 for improved resolution of DNA fragments 50-1000bp, especially primers. Higher gel strength than agarose MS6, suitable for use in blotting.

Product Image-733-2406

VWR®, Taq DNA polymerase glycerol free

Dodávateľ: VWR Chemicals
Opis: VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

Image Unavailable-733-2255

AccuStart™ II Taq DNA polymerase

Dodávateľ: Quantabio
Opis: AccuStart™ II <i>Taq</i> DNA Polymerase is a high purity, recombinant <i>Taq</i> DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified <i>Taq</i> DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

Image Unavailable-BOSSBS-11304R-CY5

Anti-RFC1 Rabbit Polyclonal Antibody (Cy5®)

Katalógové číslo: (BOSSBS-11304R-CY5)
Dodávateľ: BIOSS INC
Opis: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Merná jednotka: 1 * 100 µl
Zavolať a opýtať sa na ceny
Zásoby tejto položky sú obmedzené, no môžu sa nachádzať v inom sklade blízko pri vás. Uistite sa prosím, že ste prihlásený/á na tejto lokalite, aby sa mohli zobraziť dostupné zásoby. Ak sa stále zobrazuje zavolajte a potrebujete pomoc, zavolajte nám prosím na číslo +43 1 97002 - 0.
Zásoby tejto položky sú obmedzené, no môžu sa nachádzať v inom sklade blízko pri vás. Uistite sa prosím, že ste prihlásený/á na tejto lokalite, aby sa mohli zobraziť dostupné zásoby. Ak sa stále zobrazuje zavolajte a potrebujete pomoc, zavolajte nám prosím na číslo +43 1 97002 - 0.
Daná chemikália je regulovaná podľa platnej legislatívy a bude vyžadované vyplnenie formulára. Jeho včasným vyplnením urýchlite dodanie produktu.
-Na zakúpenie tejto položky môže byť potrebná dodatočná dokumentácia. Ak to bude potrebné, skontaktuje sa s vami zástupca spoločnosti VWR.
Tento produkt bol zablokovaný vašou organizáciou. Kontaktujte prosím vaše oddelenie nákupu pre získanie viac informácií.
Pôvodný produkt už nie je dostupný. Je dostupná zobrazená náhrada.
Tento produkt už nie je dostupný. Môžu byť k dispozícii alternatívy, ktoré sa dajú vyhľadať pomocou katalógového čísla VWR uvedeného vyššie. Ak potrebujete ďalšiu pomoc, zavolajte prosím na zákaznícky servis spoločnosti VWR na čísle +43 1 97002 - 0.
17 - 32 of 6 208
no targeter for Bottom