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Soupravy pro purifikaci PCR produktů, E.Z.N.A.® a E-Z 96® Cycle Pure

Dodávateľ: OMEGA BIOTEK
Opis: Soupravy E.Z.N.A.® Cycle Pure kit jsou navrženy pro rychlou purifikaci jedno-, případně dvouřetězcové DNA z PCR nebo jiných enzymatických reakčních směsí. Purifikační postup kompletně odstraňuje primery, nukleotidy, enzymy, soli a další nečistoty ze vzorků DNA. Soupravy E.Z.N.A.® Microelute Cycle Pure Kit jsou specificky určeny pro purifikaci PCR vzorků o malém elučním objemu od 10 do 15 µl. Souprava E-Z® 96 Cycle Pure Kit umožňuje současnou purifikaci až 96 PCR vzorků z paralelních amplifikací. Souprava E-Z® Cycle Pure Kit využívá vícejamkové technologie pro jak manuální, tak plně automatizované purifikace při vysokém počtu vzorků.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

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RNA isolation, total RNA kit, E.Z.N.A.® MicroElute

Dodávateľ: OMEGA BIOTEK
Opis: E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5×10⁵ eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10 to 15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, Poly A+ purification, nuclease protection and<i> invitro</i> translation.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

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Mag-Bind® Plant DNA plus kit

Dodávateľ: OMEGA BIOTEK
Opis: The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

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E.Z.N.A.® Tissue DNA Kit

Dodávateľ: OMEGA BIOTEK
Opis: Souprava E.Z.N.A.® tissue DNA kit nabízí jednoduchou, rychlou a cenově výhodnou metodu pro izolaci DNA ze široké škály typů vzorků, včetně čerstvých nebo zmražených živočišných buněk a tkání. Po lyzi buněk může být purifikace DNA hotová do 30 minut. Najednou může být pomocí jednoduchého E.Z.N.A.® tissue DNA protokolu snadno zpracováno až 30 mg tkáně. Souprava využívá centrifugační kolonky a umožňuje zpracovávat jeden vzorek či více vzorků současně. Nejsou nutné extrakce fenolem/chloroformem ani časově náročné kroky jako precipitace izopropanolem nebo ethanolem. DNA purifikovaná za využití soupravy E.Z.N.A.® tissue DNA kit je vhodná pro většinu následných aplikací, jako jsou PCR, southern blot a restrikční enzymatické štěpení.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

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Mag-Bind® HDQ blood and tissue DNA 96 Kit

Dodávateľ: OMEGA BIOTEK
Opis: The Mag-Bind® Blood and Tissue DNA HDQ 96 Kit is designed for rapid and reliable isolation of high quality genomic DNA from 100 to 200 μl blood samples, buccal swabs, buffy coat and cultured cells. All heating steps that limit robotic applications have been removed to allow for faster processing. Mag-Bind® particles HDQ provide quick magnetic response time reducing overall processing time. Utilising paramagnetic particles provides high quality DNA that is suitable for direct use in most downstream applications, such as qPCR, PCR and Next Generation Sequencing. The Mag-Bind® HDQ Blood DNA and Tissue 96 Kit is compatible with many robotic liquid handlers and magnetic processors, including Thermo Scientific KingFisher® Flex.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

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PCR clean-up kit, Axygen® AxyPrep™ Mag

Dodávateľ: Corning
Opis: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

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RNA isolation, total RNA kit II, E.Z.N.A.®

Dodávateľ: OMEGA BIOTEK
Opis: The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and<i> in vitro</i> translation.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

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E.Z.N.A.® blood RNA kit

Dodávateľ: OMEGA BIOTEK
Opis: The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

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Mag-Bind® Endo-Free plasmid 96 kit

Dodávateľ: OMEGA BIOTEK
Opis: The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

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E.Z.N.A.® Plasmid Midi kit

Dodávateľ: OMEGA BIOTEK
Opis: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

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RNA isolation, E.Z.N.A.® HP total RNA kit

Dodávateľ: OMEGA BIOTEK
Opis: The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1×10⁷ eukaryotic cells or 25 to 30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and<i> in vitro</i> translation.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

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E.Z.N.A.® blood DNA maxi kit

Katalógové číslo: (D2492-03)
Dodávateľ: OMEGA BIOTEK
Opis: The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 10 ml whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.
Merná jednotka: 1 * 50 Tests

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Accessories for genomic DNA purification kits

Dodávateľ: OMEGA BIOTEK
Opis: Nucleic Acid Purification Kits and Reagents, cHTR reagent, 50 ml

Product Image-PIER78870

Pierce™ DNA Extraction Reagent Kit, Yeast

Katalógové číslo: (PIER78870)
Dodávateľ: Thermo Fisher Scientific
Opis: The Pierce™ yeast DNA extraction reagent kit uses Y-PER yeast protein extraction reagent to quickly extract and purify genomic and plasmid DNA from yeast.
Merná jednotka: 1 * 1 KIT
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Accessories for E.Z.N.A.® HP total RNA kit

Katalógové číslo: (AC-1370-00)
Dodávateľ: OMEGA BIOTEK
Opis: Nucleic Acid Purification Kits and Reagents, Collection tube, 2 ml, capless, graduated
Merná jednotka: 1 * 5.000 items

Certifikáty


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Accessories for NucleoSpin® 8/96 Tissues/Tissue Core Kits

Dodávateľ: MACHEREY-NAGEL
Opis: Nucleic Acid Purification Kits and Reagents, MN positive pressure frame, 96-well plate

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