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Product Image-BE-405

Experimentation kits, enzyme analysis

Katalógové číslo: (BE-405)
Dodávateľ: G BIOSCIENCES
Opis: This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.
Merná jednotka: 1 * 1 KIT
Product Image-BE-415

Experimentation kits, ion exchange chromatography

Katalógové číslo: (BE-415)
Dodávateľ: G BIOSCIENCES
Opis: Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.
Merná jednotka: 1 * 1 KIT
Product Image-BE-301

Experimentation kits, bacterial conjugation

Katalógové číslo: (BE-301)
Dodávateľ: G BIOSCIENCES
Opis: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.
Merná jednotka: 1 * 1 KIT
Product Image-BE-507

Experimentation kits, immunoaffinity chromatography

Katalógové číslo: (BE-507)
Dodávateľ: G BIOSCIENCES
Opis: A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilised on a chromatographic resin. The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction. In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilised in research laboratories.
Merná jednotka: 1 * 1 KIT
Product Image-BE-408

Experimentation kits, protein fingerprinting

Katalógové číslo: (BE-408)
Dodávateľ: G BIOSCIENCES
Opis: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.
Merná jednotka: 1 * 1 KIT
Product Image-763-0009

Kits: the case of the invisible bands, Ready-to-Load™

Katalógové číslo: (763-0009)
Dodávateľ: EDVOTEK
Opis: Experiment using fluorescence and electrophoresis to solve the mystery of the invisible bands.
Merná jednotka: 1 * 1 items
Product Image-EDVO118-C

Kits: cholesterol diagnostics, Ready-to-Load™

Dodávateľ: EDVOTEK
Opis: Genetic testing can be used to identify people with a genetic condition which caused them to have an elevated level of cholesterol and which can be fatal. Students can see how genetic testing is carried out and learn about DNA electrophoresis.

Product Image-763-0001

Experimentation kits, amplification of DNA by PCR

Katalógové číslo: (763-0001)
Dodávateľ: EDVOTEK
Opis: In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.
Merná jednotka: 1 * 1 KIT
Product Image-BE-416

Experimentation kits, hydrophobic chromatography

Katalógové číslo: (BE-416)
Dodávateľ: G BIOSCIENCES
Opis: The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilising a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.
Merná jednotka: 1 * 1 KIT
Product Image-EDVO221

Transformation of E. coli with pGAL™

Katalógové číslo: (EDVO221)
Dodávateľ: EDVOTEK
Opis: In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the <i>lacZ</i> gene. IPTG is not required in this experiment since pGAL contains the complete <i>lacZ</i> gene.
Merná jednotka: 1 * 1 items
Image Unavailable-466-0302

Accessories for Heratherm® large capacity general protocol and advanced protocol ovens

Dodávateľ: Thermo Fisher Scientific
Opis: Stohovací sady, Heratherm®, Určeno pro: Všechny modely General Protocol, Advanced Protocol a Advanced Protocol Security, 60 l

Product Image-EDVO253

Experimentation kits, diversity of fish proteins

Katalógové číslo: (EDVO253)
Dodávateľ: EDVOTEK
Opis: In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.
Merná jednotka: 1 * 1 KIT
Image Unavailable-NEUB0500-10-33

Electrolysis apparatus according to Hoffmann

Dodávateľ: NEUBERT VOLUME GLASSWAERE
Opis: Electrolysis apparatus according to Hoffmann, made of borosilicate glass 3.3.

Product Image-BE-402

Experimentation kits, assays for protein quantification

Dodávateľ: G BIOSCIENCES
Opis: Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Product Image-EDVO316

Experimentation kits, in search of the cholesterol gene

Katalógové číslo: (EDVO316)
Dodávateľ: EDVOTEK
Opis: Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.
Merná jednotka: 1 * 1 KIT
Product Image-BE-407

Experimentation kits, protein structure analysis

Katalógové číslo: (BE-407)
Dodávateľ: G BIOSCIENCES
Opis: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.
Merná jednotka: 1 * 1 KIT
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