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Master mixes, multiplex, Phusion® U

Dodávateľ: FINNZYMES REAGENTS
Opis: Phusion® U multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Product Image-F-564S

Master mixes, green multiplex, Phusion® U

Dodávateľ: FINNZYMES REAGENTS
Opis: Phusion® U green multiplex PCR master mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Product Image-27-9559-01

PCR beads, illustra™ PuReTaq Ready-To-Go™

Dodávateľ: Cytiva
Opis: PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

Image Unavailable-ENZOADIEKS390A

PKA kinase activity kit

Katalógové číslo: (ENZOADIEKS390A)
Dodávateľ: ENZO LIFE SCIENCES
Opis: The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
Merná jednotka: 1 * 1 KIT
Image Unavailable-ENZOADIEKS420A

PKC kinase activity kit

Katalógové číslo: (ENZOADIEKS420A)
Dodávateľ: ENZO LIFE SCIENCES
Opis: The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
Merná jednotka: 1 * 1 KIT
Product Image-MAG-PCR-CL-50

PCR clean-up kit, Axygen® AxyPrep™ Mag

Dodávateľ: Corning
Opis: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

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Taq DNA polymerase, MP Biomedicals

Dodávateľ: MP BIOMEDICALS
Opis: <i>Taq</i> DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium <i>Thermus aquaticus</i>. The recombinant form of this enzyme is expressed in <i>E. coli</i>. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

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Taq DNA polymerase (recombinant)

Dodávateľ: Thermo Fisher Scientific
Opis: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant <i>Taq</i> DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.

Certifikáty

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Taq DNA polymerase (native, without BSA)

Katalógové číslo: (EP0282)
Dodávateľ: Thermo Fisher Scientific
Opis: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.
Merná jednotka: 1 * 1 KIT
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Taq DNA polymerase (native, with BSA)

Katalógové číslo: (EP0072)
Dodávateľ: Thermo Fisher Scientific
Opis: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>. Native<i> Taq</i> DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.
Merná jednotka: 1 * 1 KIT
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Pfu DNA polymerase

Dodávateľ: G BIOSCIENCES
Opis: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

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Peqlab, Components for Peqlab DNA Polymerases

Katalógové číslo: (PEQL01-1018)
Dodávateľ: VWR Peqlab
Opis: 10X PCR Buffer, incomplete
Merná jednotka: 1 * 500 mL

Certifikáty


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VWR®, Taq DNA polymerase, glycerol-free

Dodávateľ: VWR Chemicals
Opis: Highly concentrated Taq DNA polymerase glycerol free 50 U/µl is ideal for industrial use and manufactures of diagnostic PCR kits.

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Restriction enzymes, rTaqPDNA

Dodávateľ: Apollo Scientific
Opis: Recombinant Taq plus DNA polymerase produced in <i>E.coli </i>5 U/µl.

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Taq DNA polymeráza

Dodávateľ: VWR Chemicals
Opis: VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from <i>Thermus aquaticus</i>, and has a molecular weight of approximately 94 kDa. VWR® <i>Taq</i> DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

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VWR® Fast extract genotyping PCR kit

Dodávateľ: VWR Chemicals
Opis: This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

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