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Dodávateľ: G BIOSCIENCES
Opis: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Katalógové číslo: (17-0045-02)
Dodávateľ: Cytiva
Opis: Sephadex™ G-50 M DNA Grade chromatography resin for purification of DNA fragments up to 20 bases in length from small molecules such as salts by size exclusion.
Merná jednotka: 1 * 100 g


Dodávateľ: VWR Chemicals
Opis: The fast extract DNA solution provides rapid and efficient extraction of PCR-ready DNA from mammalian and plant tissues. DNA is ready in eight minutes. The one-reagent protocol is divided into two simple heating steps, which can be directly followed by PCR analysis, such as screening and genotyping.

Katalógové číslo: (H63457.06)
Dodávateľ: Thermo Fisher Scientific
Opis: Fmoc-Pro-OPfp 98%
Merná jednotka: 1 * 5 g

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Dodávateľ: OMEGA BIOTEK
Opis: <p>Souprava pro izolaci bakteriální DNA Mag-Bind® umožňuje rychlou a spolehlivou izolaci genomové DNA (gDNA) vysoké kvality z široké škály druhů bakterií. Naráz může být zpracováváno až 0,5 ml gram pozitivních či gram negativních bakteriálních kultur. Klíčem k tomuto systému jsou částice Mag-Bind®, které v reversibilním procesu naváží za jistých optimálních podmínek DNA či RNA a umožní tak odstranění proteinů či jiných kontaminantů.</p>

Katalógové číslo: (25-2020)
Dodávateľ: VWR Chemicals
Opis: peqGOLD DNA ladder 100 bp plus is ideal for size determination in the range of 100 to 3000 base pairs. There are no unspecific bands besides the fragments described. 1000 and 500 bp bands have a higher DNA content and serve as a size reference.
Merná jednotka: 1 * 1 KIT

Katalógové číslo: (733-2479)
Dodávateľ: VWR Chemicals
Opis: Taq DNA polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from thermus aquaticus and has a molecular weight of approximately 94 kDa.
Merná jednotka: 1 * 1 KIT


Katalógové číslo: (17-0574-02)
Dodávateľ: Cytiva
Opis: Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.
Merná jednotka: 1 * 100 g


Dodávateľ: G BIOSCIENCES
Opis: GET™ DNA Template is a spin column format kit suitable for the preparation of DNA templates from blood, cells, fungal and animal tissue samples. The isolated genomic DNA is suitable for PCR and other downstream applications.

Dodávateľ: AAT BIOQUEST
Opis: ReadiUse™ 100 bp DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

New Product

Dodávateľ: Thermo Fisher Scientific
Opis: DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA. They are commonly used in the studies of plasmid DNA and PCR products.
Dodávateľ: OMEGA BIOTEK
Opis: The Mag-Bind® Plant DNA 96 plus kit allows rapid and reliable isolation of high-quality genomic DNA from a wide variety of difficult to lyse and high polysaccharide plant species and tissues. The system utilises a CTAB-based lysis buffer without the need for a chloroform or other organic solvent steps. The proprietary wash buffer systems eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. The Mag-Bind® Plant DNA plus kit also provides greater flexibility than silica plate based systems as partial plates can be used and can be scaled up to a 24-well format with the appropriate magnetic stand. The streamlined protocol can be easily adapted on most robotic liquid handling platforms. Purified DNA is suitable for PCR, qPCR, restriction digestion, hybridisation applications, as well as more demanding applications, such as RAPD.
Dodávateľ: Thermo Fisher Scientific
Opis: From 250 to 5000 base pairs: 5 bands from 125 to 1000 bp; four bands from 1500 to 5000 bp. Recommended load volume 20 µl.
Dodávateľ: OMEGA BIOTEK
Opis: The Mag-Bind® Universal Pathogen DNA 96 Kit allows rapid and reliable isolation of high-quality pathogen and host genomic DNA from tissue samples. Up to a 30 mg tissue sample is recommended and can be processed in less than 60 minutes. The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments.
Dodávateľ: VWR Chemicals
Opis: VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
Dodávateľ: G BIOSCIENCES
Opis: <i>Taq</i> DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile <i>Thermus aquaticus</i>. The molecular weight of the recombinant protein is 94 kD. The <i>Taq</i> Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this <i>Taq</i> Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. <i>Taq</i> DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

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