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Hľadali ste: 5(6)-Carboxyfluorescein+N-hydroxysuccinimide+ester+(5\/6-FAM+SE)


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Dodávateľ: Thermo Fisher Scientific
Opis: Pierce™ Sulfo-NHS (N-hydroxysulfosuccinimide) is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilisation methods.
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific EZ-Link Sulfo-NHS-SS-Biotin is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Dodávateľ: Thermo Fisher Scientific
Opis: The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 to 145 nm in excitation/emission wavelengths. The DyLight™ LS dyes provide multicolour detection using a single excitation source in applications including flow cytometry, conjugation, DNA-sequencing, microscopy and fluorescence<i> in situ</i> hybridization (FISH). These dyes contain N-hydroxysuccinimide (NHS) esters, the most commonly used reactive group for labeling proteins. NHS esters react with primary amines on proteins and other molecules, forming a stable, covalent amide bond and releasing the NHS group.

Katalógové číslo: (PIERA35394)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
Merná jednotka: 1 * 10 mg


Katalógové číslo: (PIERA39266)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.
Merná jednotka: 1 * 20 mg


Katalógové číslo: (PIERA39257)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Merná jednotka: 1 * 10 mg

Certifikáty


Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Katalógové číslo: (PIER22309)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Pierce GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (7.3 angstroms).
Merná jednotka: 1 * 50 mg


Katalógové číslo: (SIAL803332-50MG)
Dodávateľ: Merck
Opis: ANB-NOS and Sulfo-SANPAH are heterobifunctional crosslinkers that contain an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. NHS esters react efficiently with primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. When exposed to UV light nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines).
Merná jednotka: 1 * 50 mg


Katalógové číslo: (SIAL803642-50MG)
Dodávateľ: Merck
Opis: The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.
Merná jednotka: 1 * 50 mg


Katalógové číslo: (786-082)
Dodávateľ: G BIOSCIENCES
Opis: Ideal for enzyme-antibody crosslinking.
Merná jednotka: 1 * 8 items


Katalógové číslo: (PIER21857)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.
Merná jednotka: 1 * 50 mg

Certifikáty


Katalógové číslo: (AATB4503)
Dodávateľ: AAT BIOQUEST
Opis: Compared to SMCC and sulfo-SMCC, SMCC Plus has enhanced water solubility and crosslinking efficiency.
Merná jednotka: 1 * 5 mg


Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Pierce Sulfo-SMCC, No-Weigh Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 angstroms).

Katalógové číslo: (PIER22589)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.
Merná jednotka: 1 * 50 mg


Katalógové číslo: (PIER22360)
Dodávateľ: Thermo Fisher Scientific
Opis: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
Merná jednotka: 1 * 50 mg

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