Hľadali ste: Nucleic Acid Purification Kits and Reagents

Dodávateľ: OMEGA BIOTEK

Opis: Selektivní štěpení proteázou uvolňuje z FFPE vzorků mikrogramová množství DNA. Purifikované nukleové kyseliny, ačkoliv vysoce fragmentované, jsou vhodné pro širokou škálu následných genomových analýz a analýz genové exprese. Soupravy E.Z.N.A.® obsahující centrifugační kolonky jsou ideální pro nízký počet vzorků, zatímco Mag-Bind® soupravy fungující na principu magnetických kuliček jsou navrženy speciálně pro uživatele s vysokým počtem vzorků s možností automatizace.

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Dodávateľ: OMEGA BIOTEK

Opis: Souprava E.Z.N.A.® Water DNA Kit je navržena pro izolaci vysoce čisté buněčné DNA z vodních vzorků obsahujících huminové kyseliny a inhibitory PCR. Tato souprava je velice účinná při izolaci DNA z bakterií, u kterých se těžko provádi lyze, z hub a z řas, které se nachází ve vodě z vodovodního řádu, jezerech, řekách a v kanalizaci. DNA purifikovaná pomocí soupravy E.Z.N.A.® Water DNA Kit je připravena pro následné aplikace, jako jsou PCR, Southern blotting a anylýza SNP.

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Katalógové číslo: (OMEGM6399-01PF96)

Dodávateľ: OMEGA BIOTEK

Opis: Automation-ready, prefilled 96-well plates for quick and convenient DNA extraction from blood, saliva, cultured cells, and swabs for magnetic processors.

Merná jednotka: 1 * 1 KIT

Predaj

Dodávateľ: OMEGA BIOTEK

Opis: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free plasmid mini kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<0,1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

Dodávateľ: OMEGA BIOTEK

Opis: The Mag-Bind® Stool DNA 96 Kit allows rapid and reliable isolation of high-quality genomic DNA (both pathogen and host DNA) from stool samples.

Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Total RNA kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the E.Z.N.A.® Total RNA kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the E.Z.N.A.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and<i> in vitro</i> translation.

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Dodávateľ: OMEGA BIOTEK

Opis: The MicroElute RNA clean-up kit is designed to purify and concentrate RNA (from 50 μg down to picogram amounts) from enzymatic reactions or for desalting RNA samples (e.g. following phenol extraction and ethanol precipitation). The procedure uses lower elution volumes, making it well suited for applications require highly concentrated RNA sample.

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Dodávateľ: OMEGA BIOTEK

Opis: Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample.

Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, <i>E.coli</i> strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription<i> invitro</i>, and other applications.

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Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

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Dodávateľ: OMEGA BIOTEK

Opis: E.Z.N.A.® Plant RNA kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogeniser column for filtration and homogenisation of viscous plant cell lysate by centrifugation in combination with the HiBind® RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, northern blot analysis, differential display, and poly(A)+ RNA selection.

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Dodávateľ: OMEGA BIOTEK

Opis: Soupravy E.Z.N.A.® Fastfilter® Plazmid Kit rychle purifikují plazmidovou DNA pomocí stříkaček s filtrem na promytí lyzátu, soupravy E-Z 96® Fastfilter Plasmid kit využívají pro čištění lyzátu destičky v 96-jamkovém formátu. Soupravy Fastfilter® umožnují izolaci plazmidu v midi- a maxi- měřítku během méně než 40 minut.

Katalógové číslo: (786-132)

Dodávateľ: G BIOSCIENCES

Opis: The GET™ Total RNA Kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA spin columns. After homogenisation, RNA is bound to the GET™ RNA spin columns and washed. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays.

Merná jednotka: 1 * 1 KIT

Predaj

Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Universal Pathogen Kit allows for the rapid and reliable isolation of high quality host genomic DNA, gram positive and negative bacterial DNA, fungal spore DNA and viral DNA and viral RNA from tissue, urine, serum and fecal samples.

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Dodávateľ: OMEGA BIOTEK

Opis: The Mag-Bind® Blood and Tissue DNA HDQ 96 Kit is designed for rapid and reliable isolation of high quality genomic DNA from 100 to 200 μl blood samples, buccal swabs, buffy coat and cultured cells. All heating steps that limit robotic applications have been removed to allow for faster processing. Mag-Bind® particles HDQ provide quick magnetic response time reducing overall processing time. Utilising paramagnetic particles provides high quality DNA that is suitable for direct use in most downstream applications, such as qPCR, PCR and Next Generation Sequencing. The Mag-Bind® HDQ Blood DNA and Tissue 96 Kit is compatible with many robotic liquid handlers and magnetic processors, including Thermo Scientific KingFisher® Flex.

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Dodávateľ: OMEGA BIOTEK

Opis: <p>Souprava pro izolaci bakteriální DNA Mag-Bind® umožňuje rychlou a spolehlivou izolaci genomové DNA (gDNA) vysoké kvality z široké škály druhů bakterií. Naráz může být zpracováváno až 0,5 ml gram pozitivních či gram negativních bakteriálních kultur. Klíčem k tomuto systému jsou částice Mag-Bind®, které v reversibilním procesu naváží za jistých optimálních podmínek DNA či RNA a umožní tak odstranění proteinů či jiných kontaminantů.</p>