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Hľadali ste: Činidla pro extrakci bílkovin

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Katalógové číslo: (DGA01)
Dodávateľ: G BIOSCIENCES
Opis: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.
Merná jednotka: 1 * 500 items


Katalógové číslo: (786-793)
Dodávateľ: G BIOSCIENCES
Opis: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.
Merná jednotka: 1 * 5 mL


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Katalógové číslo: (786-598)
Dodávateľ: G BIOSCIENCES
Opis: Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.
Merná jednotka:


Katalógové číslo: (786-244)
Dodávateľ: G BIOSCIENCES
Opis: SilverOUT removes interfering silver ions from gel pieces.
Merná jednotka: 1 * 100 Tests


Katalógové číslo: (786-681)
Dodávateľ: G BIOSCIENCES
Opis: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.
Merná jednotka: 1 * 1 KIT


Dodávateľ: ENZO LIFE SCIENCES
Opis: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

New Product

Katalógové číslo: (786-241)
Dodávateľ: G BIOSCIENCES
Opis: InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.
Merná jednotka: 1 * 1 KIT


Dodávateľ: G BIOSCIENCES
Opis: Protein-S-S-Reductant™ uses TCEP (Tris [2-carboxyethyl] phosphine), a popular alternative to β-mercaptoethanol and DTT (dithiothreitol). TCEP improves stability, increases effectiveness, and reduces proteins over a wider range of pH, including lower acidic pHs. Protein-S-S-Reductant™ completely reduces stable disulfide bonds in less than 5 minutes at room temperature and is compatible with the protein alkylation reactions. This ready to use solution is at a neutral pH and stabilised for long-term storage. Simply supplement Protein-S-S-Reductant™ in place of DTT or β-mercaptoethanol and boil the sample.

Katalógové číslo: (ENZOBMLKI1761250)
Dodávateľ: ENZO LIFE SCIENCES
Opis: FLUOR DE LYS® Developer II is an essential reagent for signal generation in assays done with the FLUOR DE LYS®-HDAC8, FLUOR DE LYS®-SIRT1 and FLUOR DE LYS®-SIRT2 substrates. Deacetylation of the FLUOR DE LYS® substrate sensitises it so that, in a second step, treatment with the FLUOR DE LYS® Developer II produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1X Developer dilution (e.g. nicotinamide for sirtuins).
Merná jednotka: 1 * 1,25 mL

New Product


Dodávateľ: Thermo Fisher Scientific
Opis: The Pierce™ Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.
Katalógové číslo: (PIER90067)
Dodávateľ: Thermo Fisher Scientific
Opis: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
Merná jednotka: 1 * 1 KIT


Dodávateľ: G BIOSCIENCES
Opis: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Katalógové číslo: (ENZOBMLKI1050300)
Dodávateľ: ENZO LIFE SCIENCES
Opis: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
Merná jednotka: 1 * 300 µl

New Product


Katalógové číslo: (PIER90054)
Dodávateľ: Thermo Fisher Scientific
Opis: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
Merná jednotka: 1 * 50 µG

Katalógové číslo: (PIER90056)
Dodávateľ: Thermo Fisher Scientific
Opis: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
Merná jednotka: 1 * 100 µG


Katalógové číslo: (PIER90053)
Dodávateľ: Thermo Fisher Scientific
Opis: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
Merná jednotka: 1 * 2 µG

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