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Hľadali ste: Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.


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Product Image-PIER34096

Protein immunodetection reagents, SuperSignal™ West Femto maximum sensitivity substrate

Dodávateľ: Thermo Fisher Scientific
Opis: SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.

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Product Image-PIER37552

Peroxidase conjugate stabiliser/diluent, Guardian™

Dodávateľ: Thermo Fisher Scientific
Opis: Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

Product Image-PIER35055

Western blotting kit, ECL substrate, Pierce™

Dodávateľ: Thermo Fisher Scientific
Opis: The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Product Image-PIER34035

Substrate for western blotting, NBT (nitro-blue tetrazolium chloride)

Katalógové číslo: (PIER34035)
Dodávateľ: Thermo Fisher Scientific
Opis: NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.
Merná jednotka: 1 * 1 g
Product Image-PIER34042

Substrate for western blotting, 1-Step™ NBT/BCIP

Katalógové číslo: (PIER34042)
Dodávateľ: Thermo Fisher Scientific
Opis: The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and <i>in situ</i> hybridisation applications.
Merná jednotka: 1 * 250 mL
Image Unavailable-PIER15162

NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™

Katalógové číslo: (PIER15162)
Dodávateľ: Thermo Fisher Scientific
Opis: QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
Merná jednotka: 1 * 1 KIT
Image Unavailable-786-423

High molecular weight transfer buffer (5X)

Katalógové číslo: (786-423)
Dodávateľ: G BIOSCIENCES
Opis: High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
Merná jednotka: 1 * 1 L
Product Image-PIER34070

Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor

Katalógové číslo: (PIER34070)
Dodávateľ: Thermo Fisher Scientific
Opis: The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and <i>in situ</i> hybridisation applications.
Merná jednotka: 1 * 100 mL
Product Image-PIER34000

CN/DAB substrate kit, Pierce™

Katalógové číslo: (PIER34000)
Dodávateľ: Thermo Fisher Scientific
Opis: Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
Merná jednotka: 1 * 1 KIT
Product Image-PIER34012

1-Step™, Pierce™ Chloronaphthol

Katalógové číslo: (PIER34012)
Dodávateľ: Thermo Fisher Scientific
Opis: Chloronaphthol (4-CN) is a peroxidase substrate used for chromogenic detection of HRP in immunoblotting and immunohistochemical applications. This product is less sensitive than other precipitating substrates, but the distinct blue to blue-purple precipitate photographs well and can be useful in double-staining applications. The coloured product is soluble in alcohol and xylenes, but is not soluble in aqueous buffers. The 1-Step Chloronaphthol is supplied ready to use.
Merná jednotka: 1 * 250 mL
Product Image-PIER37574

1-Step™ Ultra TMB-Blotting solution, Pierce™

Katalógové číslo: (PIER37574)
Dodávateľ: Thermo Fisher Scientific
Opis: The Thermo Scientific 1-Step Ultra TMB-Blotting Solution is a ready-to-use, single-component horseradish peroxidase (HRP) substrate for Western blotting applications (not for use in ELISA assays or immunohistochemical applications). The high sensitivity, increased signal-to-noise ratio and low background blotting solution contains soluble TMB (3,3´,5,5´-tetramethylbenzidine), which reacts with HRP to produce an insoluble dark blue precipitate. The substrate is compatible with both nitrocellulose and PVDF membranes.
Merná jednotka: 1 * 250 mL
Product Image-AATB26101

Amplite® West ECL HRP Substrate *Femto Sensitivity*

Dodávateľ: AAT BIOQUEST
Opis: Amplite® West ECL HRP substrate is an ultra-sensitive chemiluminescent (ECL) substrate formulated for highest sensitivity and stability.

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Product Image-PIER34075

Protein immunodetection reagents, SuperSignal™ West Dura extended duration substrate

Dodávateľ: Thermo Fisher Scientific
Opis: The SuperSignal™ West Dura Extended Duration Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal West Dura Substrate enables mid-femtogram detection of antigen by oxidising luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualised on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions.

Product Image-PIER20148X

Chemiluminescent EMSA kit, LightShift™

Dodávateľ: Thermo Fisher Scientific
Opis: LightShift™ Chemiluminescent EMSA Kit is a robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterise protein-DNA binding interactions.

Product Image-PIER34002

DAB substrate

Katalógové číslo: (PIER34002)
Dodávateľ: Thermo Fisher Scientific
Opis: DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.
Merná jednotka: 1 * 1 KIT
Product Image-PIER17075

Biotin random prime DNA labelling kit, North2South™

Katalógové číslo: (PIER17075)
Dodávateľ: Thermo Fisher Scientific
Opis: North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
Merná jednotka: 1 * 1 KIT
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