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Kryštálová violeť ethanolic aqueous solution primary stain for Gram staining, Sigma-Aldrich®

Dodávateľ: Merck
Varování

Synonymá: CI 42555

1.09218.0500 1.09218.2500
1.09218.0500EA 52.87 EUR
1.09218.0500 1.09218.2500
Kryštálová violeť ethanolic aqueous solution primary stain for Gram staining, Sigma-Aldrich®
Kryštálová violeť

The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.


The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.

Vzorec: C₂₅H₃₀ClN₃
Mw: 407.99
Skladovacia teplota: Ambient
Číslo MDL: MFCD00011750
Číslo CAS: 548-62-9
OSN: 1170
ADR: 3 III
Merck Index: 13,04406
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