Ubiquilin 2 (also known as PLIC2 and Chap1) is a member of the ubiquilin protein family, which regulate the degradation of cellular proteins through proteasome or autophage-like pathways. Humans have four ubiquilin genes, each encoding a separate protein referred to as Ubiquilin 1, 2, 3 and 4. All ubiquilins contain an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain, while the central part of the molecules are highly variable. Ubiquilin 2 protein is of different molecular size in mouse and human, 638 and 624 amino acids respectively. As a result the mouse protein, endogenously expressed in rodent 3T3 cells, runs on SDS-PAGE and western blots slightly slower than the human protein.
Application information:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: UBQL2
Clonality: Monoclonal
Clone: 6H9
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human, Mouse
![Left: Ubiquilin 2 expression (green) in NIH-3T3 cells. Red: chicken anti-lamin A/C. Right: WB of ubiquilin 2 (green). [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y, [7] rat whole brain, and [8] mouse whole brain.](https://sk.vwr.com/stibo/low_res/std.lang.all/51/49/26685149.jpg "Left: Ubiquilin 2 expression (green) in NIH-3T3 cells. Red: chicken anti-lamin A/C. Right: WB of ubiquilin 2 (green). [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y, [7] rat whole brain, and [8] mouse whole brain.")
![Left: Ubiquilin 2 expression (green) in NIH-3T3 cells. Red: chicken anti-lamin A/C. Right: WB of ubiquilin 2 (green). [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y, [7] rat whole brain, and [8] mouse whole brain.](https://sk.vwr.com/stibo/grid/std.lang.all/51/49/26685149.jpg)
