Clelandovo činidlo ≥99.4% for biotechnology
Dodávateľ: VWR Chemicals
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Varování
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0281-25GEA
555.2
EUR
0281-100G
0281-25G
0281-1KG
0281-5G
0281-2500
Clelandovo činidlo ≥99.4% for biotechnology
Clelandovo činidlo
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
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Vzorec:
C₄H₁₀O₂S₂ Mw: 154,25 g/mol Teplota varu: < 125 °C (1013 hPa) Teplota tavenia: 42…44 °C Teplota vzplanutia: >110 °C (uz. pohárek) Skladovacia teplota: Refrigerator |
Číslo MDL:
MFCD00004877 Číslo CAS: 3483-12-3 EINECS: 222-468-7 |
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Výsledky špecifikačného testu
| Abs.@283 nm (0.02 M, Water) | ≤0.05 |
| DNase | none detected |
| Loss on Drying | <0.5% |
| Melting Point | 39 – 43 °C |
| Oxidised DTT | <0.5% |
| Protease | none detected |
| Purity (-SH Content) | >99.4% |
| RNase | none detected |
| Solubility (5%, Water) | pass |
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