Katalógové číslo: (786-022)

Dodávateľ: G BIOSCIENCES

Opis: FOCUS™ Mitochondria kit is specifically designed for the isolation of intact mitochondria from cultured mammalian cells. This kit allows for the fast and efficient fractionation of the cytoplasm of cultured mammalian cells into an enriched fraction of mitochondria. The majority (>90%) of the mitochondria have intact inner and outer membranes and therefore retain their functionality.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-258)

Dodávateľ: G BIOSCIENCES

Opis: Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilises nearly all of the proteins from <i>E.coli</i>, including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife™ Lysozyme enzyme, followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

Merná jednotka: 1 * 1 KIT

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Dodávateľ: G BIOSCIENCES

Opis: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.

Dodávateľ: G BIOSCIENCES

Opis: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Certifikáty

Dodávateľ: G BIOSCIENCES

Opis: The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Katalógové číslo: (ENZOENZ450140100)

Dodávateľ: ENZO LIFE SCIENCES

Opis: Rapid, reliable method to isolate histone proteins in mammalian cells and tissues for histone modification applications.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-247)

Dodávateľ: G BIOSCIENCES

Opis: FOCUS™ Soluble & Insoluble is a complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The kit comes with reagents necessary for fractionation of soluble and insoluble fractions, including a strong chaotropic extraction buffer to solubilise difficult proteins.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-253)

Dodávateľ: G BIOSCIENCES

Opis: FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-249)

Dodávateľ: G BIOSCIENCES

Opis: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-257)

Dodávateľ: G BIOSCIENCES

Opis: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-244)

Dodávateľ: G BIOSCIENCES

Opis: SilverOUT removes interfering silver ions from gel pieces.

Merná jednotka: 1 * 100 Tests

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Dodávateľ: ENZO LIFE SCIENCES

Opis: Antigen retrieval reagent is used to recover antigens masked by fixation in cross linking fixatives such as formalin.

New Product

Katalógové číslo: (786-681)

Dodávateľ: G BIOSCIENCES

Opis: InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-793)

Dodávateľ: G BIOSCIENCES

Opis: Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave Immunoglobulin G (IgG) molecules in the hinge region.

Merná jednotka: 1 * 5 mL

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Dodávateľ: G BIOSCIENCES

Opis: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Katalógové číslo: (DGA01)

Dodávateľ: G BIOSCIENCES

Opis: The 'critical micelle concentration' (CMC) of a detergent varies with temperature, pH, ionic strength, detergent concentration, purity and presence of organic agents in the detergent. Using a large excess of detergent may pose problems during purification procedures or other downstream applications.

Merná jednotka: 1 * 500 items

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