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Opis: The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Dodávateľ: TriLink BioTechnologies

Opis: EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko <em>et al.</em> showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.

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Opis: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Opis: Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.

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Opis: CleanCap® Reagent AU is designed for the co-transcriptional capping of self-replicating or self-amplifying (saRNA) to produce an optimal Cap 1.

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Opis: Inorganic Pyrophosphatase (iPPase) is an enzyme to remove pyrophosphate in IVT to drive the reaction forward.

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Opis: <p>mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in <em>Discosoma </em>sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.</p>

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Opis: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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Opis: NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyses recombination between two loxP sites.

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Opis: T7 RNA Polymerase is a wild-type enzyme to synthesize RNA from DNA templates by <em>in vitro</em> transcription.

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Opis: This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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Dodávateľ: TriLink BioTechnologies

Opis: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Dodávateľ: TriLink BioTechnologies

Opis: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Opis: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest, and the Cas9 protein is employed to perform double-stranded DNA cleavage.

Dodávateľ: TriLink BioTechnologies

Opis: This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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Opis: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.