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Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Katalógové číslo: (M334-100ML)
Dodávateľ: VWR Chemicals
Opis: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.
Merná jednotka: 1 * 100 mL


Dodávateľ: Avantor
Opis: J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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Dodávateľ: G BIOSCIENCES
Opis: Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including <i>E.coli</i> strains.

Dodávateľ: OMEGA BIOTEK
Opis: The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.
Katalógové číslo: (ENZOADI801339)
Dodávateľ: ENZO LIFE SCIENCES
Opis: Cell lysis buffer 4, Enzo Life Sciences
Merná jednotka: 1 * 100 mL

New Product


Katalógové číslo: (ENZOADI801496)
Dodávateľ: ENZO LIFE SCIENCES
Opis: The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.
Merná jednotka: 1 * 100 mL

New Product


Katalógové číslo: (TNB-4300-L100)
Dodávateľ: TONBO BIOSCIENCE
Opis: RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.
Merná jednotka: 1 * 100 mL

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Dodávateľ: ENZO LIFE SCIENCES
Opis: Concentrated lysis buffer for preparation of cell and tissue extracts.

New Product

Dodávateľ: Avantor
Opis: J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.
Katalógové číslo: (ENZOADI801284)
Dodávateľ: ENZO LIFE SCIENCES
Opis: RIPA cell lysis buffer 2, Enzo Life Sciences
Merná jednotka: 1 * 100 mL

New Product


Dodávateľ: G BIOSCIENCES
Opis: These DNase- and RNase-free buffers and reagents are designed for high quality research.

Katalógové číslo: (ENZOBMLKI1170030)
Dodávateľ: ENZO LIFE SCIENCES
Opis: Caspase cell lysis buffer, Enzo Life Sciences
Merná jednotka: 1 * 30 mL

New Product


Dodávateľ: G BIOSCIENCES
Opis: Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Dodávateľ: AAT BIOQUEST
Opis: Current demands for RT-PCR testing of SARS-CoV-2 has led to a worldwide shortage of RNA extraction kits.

Dodávateľ: G BIOSCIENCES
Opis: Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Katalógové číslo: (786-180)
Dodávateľ: G BIOSCIENCES
Opis: Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
Merná jednotka: 1 * 500 mL


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