Hľadali ste: Protein Extraction Kits

Dodávateľ: G BIOSCIENCES

Opis: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Dodávateľ: G BIOSCIENCES

Opis: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

Dodávateľ: Thermo Fisher Scientific

Opis: B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.

Katalógové číslo: (ENZOENZ450160100)

Dodávateľ: ENZO LIFE SCIENCES

Opis: Rapid, reliable method to isolate nuclear proteins in mammalian cells and tissues.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-246)

Dodávateľ: G BIOSCIENCES

Opis: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-018)

Dodávateľ: G BIOSCIENCES

Opis: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (PIER88279)

Dodávateľ: Thermo Fisher Scientific

Opis: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-249)

Dodávateľ: G BIOSCIENCES

Opis: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-257)

Dodávateľ: G BIOSCIENCES

Opis: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.

Merná jednotka: 1 * 1 KIT

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Dodávateľ: Thermo Fisher Scientific

Opis: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).

Dodávateľ: G BIOSCIENCES

Opis: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG₁ Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.

Katalógové číslo: (786-231)

Dodávateľ: G BIOSCIENCES

Opis: The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-182)

Dodávateľ: G BIOSCIENCES

Opis: The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (PIER89899)

Dodávateľ: Thermo Fisher Scientific

Opis: The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (786-230)

Dodávateľ: G BIOSCIENCES

Opis: FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.

Merná jednotka: 1 * 1 KIT

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Katalógové číslo: (PIER84868)

Dodávateľ: Thermo Fisher Scientific

Opis: Pierce™ High pH Reversed-Phase Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.

Merná jednotka: 1 * 1 KIT

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