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Product Image-K1232

PCR cloning kit, CloneJET

Dodávateľ: Thermo Fisher Scientific
Opis: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-EL0016

T4 DNA ligase

Katalógové číslo: (EL0016)
Dodávateľ: Thermo Fisher Scientific
Opis: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
Merná jednotka: 1 * 1 KIT

Certifikáty


Product Image-27-0361-01

T4 DNA ligase

Katalógové číslo: (27-0361-01)
Dodávateľ: Cytiva
Opis: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
Merná jednotka: 1 * 50 Tests
Image Unavailable-70573-3

Ligation premix, Clonables™

Katalógové číslo: (70573-3)
Dodávateľ: NOVAGENE
Opis: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.
Merná jednotka: 1 * 55 µl
Product Image-SYGEGA1100-4XS

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Dodávateľ: SYNTHETIC GENOMICS
Opis: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Product Image-K1261

LIC cloning and expression kits, aLICator™, Fermentas

Dodávateľ: Thermo Fisher Scientific
Opis: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Product Image-GA1100-50

DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Dodávateľ: SYNTHETIC GENOMICS
Opis: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

Product Image-GA1200-50

Gibson Assembly® Ultra kit

Dodávateľ: SYNTHETIC GENOMICS
Opis: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

Product Image-GA1200-10MM

Gibson Assembly® Ultra, Master mixes

Dodávateľ: SYNTHETIC GENOMICS
Opis: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

Product Image-GA1100-10MM

Gibson Assembly® HiFi 1 Step, Master mixes

Dodávateľ: SYNTHETIC GENOMICS
Opis: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com

Product Image-216-2331

VWR®, Accessories for VWR® 24-Hour Urine Container

Dodávateľ: VWR Collection
Opis: Urine straws, 90 mm long, VWR®

Image Unavailable-BOSSBS-11043R-HRP

Anti-BEST4 Rabbit Polyclonal Antibody (HRP (Horseradish Peroxidase))

Katalógové číslo: (BOSSBS-11043R-HRP)
Dodávateľ: BIOSS INC
Opis: Bestrophin-4 is a 473 amino acid member of the Bestrophin family of proteins. Members of the Bestrophin family are transmembrane proteins that contain a high percentage of aromatic residues, a conserved RFP (Arg-Phe-Pro) motif and they function as anion channels. Bestrophin-4 acts as a calcium-sensitive chloride channel located in the cell membrane. It is believed that Bestrophin-4 also acts as a channel for other physiologically significant anions, such as bicarbonate. Bestrophin-4 is predominantly expressed in the colon, but can be found at low levels in testis, placenta, trachea, spinal chord, lung and retina.
Merná jednotka: 1 * 100 µl
Image Unavailable-BOSSBS-7613R-CY5.5

Anti-RNF144B Rabbit Polyclonal Antibody (Cy5.5®)

Katalógové číslo: (BOSSBS-7613R-CY5.5)
Dodávateľ: BIOSS INC
Opis: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.Tissue specificity: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
Merná jednotka: 1 * 100 µl
Image Unavailable-BOSSBS-7613R-CY3

Anti-RNF144B Rabbit Polyclonal Antibody (Cy3®)

Katalógové číslo: (BOSSBS-7613R-CY3)
Dodávateľ: BIOSS INC
Opis: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.Tissue specificity: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
Merná jednotka: 1 * 100 µl
Image Unavailable-BOSSBS-7613R

Anti-RNF144B Rabbit Polyclonal Antibody

Katalógové číslo: (BOSSBS-7613R)
Dodávateľ: BIOSS INC
Opis: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.Tissue specificity: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
Merná jednotka: 1 * 100 µl
Image Unavailable-BOSSBS-11043R-A488

Anti-BEST4 Rabbit Polyclonal Antibody (Alexa Fluor® 488)

Katalógové číslo: (BOSSBS-11043R-A488)
Dodávateľ: BIOSS INC
Opis: Bestrophin-4 is a 473 amino acid member of the Bestrophin family of proteins. Members of the Bestrophin family are transmembrane proteins that contain a high percentage of aromatic residues, a conserved RFP (Arg-Phe-Pro) motif and they function as anion channels. Bestrophin-4 acts as a calcium-sensitive chloride channel located in the cell membrane. It is believed that Bestrophin-4 also acts as a channel for other physiologically significant anions, such as bicarbonate. Bestrophin-4 is predominantly expressed in the colon, but can be found at low levels in testis, placenta, trachea, spinal chord, lung and retina.
Merná jednotka: 1 * 100 µl
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