Hľadali ste: FRITSCH

Dodávateľ: Thermo Fisher Scientific

Opis: DreamTaq Green PCR Master Mix (2X) is a ready to use solution containing DreamTaq™ DNA polymerase, optimised DreamTaq Green buffer, MgCl₂ and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of the PCR product on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications, such as DNA sequencing, ligation and restriction digestion. The master mix retains all features of DreamTaq DNA polymerase. It is capable for robust amplification up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Certifikáty

Dodávateľ: OMEGA BIOTEK

Opis: Díky přizpůsobení Hi-Bind® technologie 96-jamkovým destičkám nabízí souprava E-Z 96® Tissue DNA Kit vysoce výkonnou metodu purifikace genomové DNA z krve, bukálních stěrů, myšího ocasu, krysího ocasu, tkání a živočišných buněk a tkání ve formátu 96-jamkové destičky. Purifikovaná DNA je vhodná pro většinu následných aplikací, jako jsou PCR, restrikční enzymatické štěpení a hybridizační techniky.

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Dodávateľ: ENZO LIFE SCIENCES

Opis: These ddUTPs are used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. ddUTP also serves as a substrate for <i>E. coli</i> DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase.

Dodávateľ: Thermo Fisher Scientific

Opis: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Dodávateľ: Thermo Fisher Scientific

Opis: SMART Digest™ Chymotrypsin Kits are designed to provide a complimentary digestion option to the SMART Digest™ Trypsin Kits which incorporate trypsin functionality. The Chymotrypsin enzyme functionality allows digestion of proteins at different points of the amino acid chain compared to trypsin. This allows for different/additional structural information to be obtained when trying to characterise a protein. This option can also be used if a protein does not contain the specific point where trypsin cuts the protein chain.

Dodávateľ: OMEGA BIOTEK

Opis: The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Katalógové číslo: (D3392-02)

Dodávateľ: OMEGA BIOTEK

Opis: Souprava E.Z.N.A.® blood DNA kit nabízí rychlou izolaci celkové DNA z 1 až 250 µl čerstvé nebo zmražené antikoagulované plné krve. Souprava E.Z.N.A.® blood DNA kit může být též použita pro přípravu genomové DNA z buffy coatu, séra, plazmy, kostní dřeně, lymfocytů, krevních destiček a tělních tekutin. Souprava umožňuje izolace jednoho vzorku či více vzorků současně do 30 minut. Izolace probíhá bez extrakce fenolem/chloroformem a časově náročných kroků jako precipitace izopropanolem nebo ethanolem. DNA purifikovaná použitím soupravy E.Z.N.A.® blood DNA kit je vhodná pro aplikace, jako jsou PCR, southern blot nebo restrikční enzymové štěpení.

Merná jednotka: 1 * 200 Tests

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Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Plasmid Maxi Kit is the maxi scale system to deliver high-quality plasmid DNA using the spin column format, without costly accessories. The HiBind® DNA Maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. Although yields vary according to plasmid copy number, <i>E.coli</i> strain, and conditions of growth, 50 to 200 ml of overnight culture in LB medium typically produces 0,5 to 1 mg of high copy number plasmid DNA. Up to 500 ml of culture may be processed when working with low copy number plasmid. The product is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, transfection of mammalian cells, transcription<i> invitro</i>, and other applications.

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Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

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Dodávateľ: OMEGA BIOTEK

Opis: The Mag-Bind® PX blood RNA kit allows for the isolation of total RNA from 2,5 ml of whole blood that has been stored in PAXgene® Blood RNA tubes. Stabilised blood samples are first spun down and the nucleic acid pellets are washed and recollected. After washing, the nucleic acid pellet is dissolved into a specially formulated resuspension buffer. At this point the samples can be transferred to 96-well plates for processing in robotic liquid handlers and magnetic processors. High quality intact RNA can be isolated in less than 90 minutes.

Dodávateľ: Thermo Fisher Scientific

Opis: VersiPlates comprise strips of eight tubes linked to each other forming the familiar 12×8 or 96-well ANSI/SBS format. The small tear points between strips enable a single strip or as many as required for the individual experiment to be separated.

Dodávateľ: OMEGA BIOTEK

Opis: This second generation plasmid miniprep kit yields 40 to 70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 ml to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

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Katalógové číslo: (US79050)

Dodávateľ: Cytiva

Opis: The ExoProStar™ S PCR and Sequence Reaction Clean-Up Kit updates existing enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs. illustra ExoProStar™ S improves digestion efficiency with no degradation of the target PCR product. Sample remain completely intact and ready for immediate use in manual or automated processes. For maximum flexibility the enzymes are supplied in two separate tubes.

Merná jednotka: 1 * 1 KIT

Predaj

Dodávateľ: OMEGA BIOTEK

Opis: Souprava E.Z.N.A.® tissue DNA kit nabízí jednoduchou, rychlou a cenově výhodnou metodu pro izolaci DNA ze široké škály typů vzorků, včetně čerstvých nebo zmražených živočišných buněk a tkání. Po lyzi buněk může být purifikace DNA hotová do 30 minut. Najednou může být pomocí jednoduchého E.Z.N.A.® tissue DNA protokolu snadno zpracováno až 30 mg tkáně. Souprava využívá centrifugační kolonky a umožňuje zpracovávat jeden vzorek či více vzorků současně. Nejsou nutné extrakce fenolem/chloroformem ani časově náročné kroky jako precipitace izopropanolem nebo ethanolem. DNA purifikovaná za využití soupravy E.Z.N.A.® tissue DNA kit je vhodná pro většinu následných aplikací, jako jsou PCR, southern blot a restrikční enzymatické štěpení.

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Katalógové číslo: (732-2590)

Dodávateľ: Thermo Fisher Scientific

Opis: VersiPlates comprise strips of eight tubes linked to each other forming the familiar 12×8 or 96-well ANSI/SBS format. The small tear points between strips enable a single strip or as many as required for the individual experiment to be separated.

Merná jednotka: 1 * 25 items

Predaj

Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1 to 5 ml bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: Bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing. E.Z.N.A.® Plasmid Mini Kit I is available in either V-spin or Q-spin column format. V-spin column formats have an attached lid while Q-spin columns do not. Both versions can be used for centrifugation or vacuum protocols.

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