Hľadali ste: Doplňky a přísady médií z buněčných kultur

Katalógové číslo: (536252S)

Dodávateľ: VWR Chemicals

Opis: Egg yolk tellurite emulsion, VWR®

Merná jednotka: 1 * 50 mL

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Dodávateľ: VWR Chemicals

Opis: Contains a mixture of protease inhibitors: AEBSF, Aprotinin, E-64, Bestatin and Leupeptin. Each vial can be reconstituted in 1 ml deionised water to form a 100X solution.

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Dodávateľ: VWR Chemicals

Opis: Yeast extract is the water soluble portion of autolysed yeast containing a source of Vitamin B complex.

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Dodávateľ: VWR Chemicals

Opis: VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

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Dodávateľ: BIOWEST

Opis: L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

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Dodávateľ: PROTIDE PHARMACEUTICALS

Opis: Serum replacement with similar properties to TCM™ but contains additional components for cell types that have required higher concentrations of serum (typically more than 10%). TM-235™ is defined and multi-purpose. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and recombinant insulin.

Dodávateľ: HyClone products (Cytiva)

Opis: With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

Dodávateľ: G BIOSCIENCES

Opis: The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

Dodávateľ: G BIOSCIENCES

Opis: Protease-PhosphataseArrest™ provides full protection of protein samples from proteases and phosphatases released during the preparation of cell and tissue lysates. Protease-PhosphataseArrest™ contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases along with five phosphatase inhibitors that target all the phosphatase categories: serine/threonine (Ser/Thr) specific, tyrosine specific and dual specificity phosphatases.

Dodávateľ: HyClone products (Cytiva)

Opis: HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.

Katalógové číslo: (733-1948)

Dodávateľ: G BIOSCIENCES

Opis: A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Plant ProteaseArrest™ inhibits plant serine, cysteine and other plant specific proteases including aminopeptidases, aspartic and metalloproteases.

Merná jednotka: 1 * 1 mL

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Dodávateľ: MP BIOMEDICALS

Opis: M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of<i> E.coli</i> and plasmid amplification.

Katalógové číslo: (786-207)

Dodávateľ: G BIOSCIENCES

Opis: Protease inhibitor set contains 12 ready to use individual protease inhibitors, each at a 100X concentration. Various concentrations and/or combinations of protease inhibitors may be used to inhibit a broad spectrum of protease activity. The Protease inhibitor set can be used to design specific protease inhibitor cocktails, supplement existing cocktails or to screen for specific protease classes.

Merná jednotka: 1 * 1 SET

Predaj

Katalógové číslo: (733-1945)

Dodávateľ: G BIOSCIENCES

Opis: A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

Merná jednotka: 1 * 1 mL

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Dodávateľ: ENZO LIFE SCIENCES

Opis: Natural products are an unsurpassed source of chemical diversity and an ideal starting point for any screening program for pharmacologically active small molecules. Historically, natural products have been the most successful source of new drugs. The Screen-Well® natural product library offers over 500 isolated natural compounds of known structure and includes many compounds from ethnopharmacology. Compounds are provided in solution in DMSO. Individual compounds or subsets can be resupplied in gram quantities.

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Dodávateľ: PROVITRO

Opis: Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the passage kit. After thawing store the passage kit in the dark at 2 °C to 8 °C for a maximum of 4 weeks. Take care: After thawing shelf time is limited to 4 weeks.
After thawing the passage kit can be stored in the dark at 2 °C to 8 °C for up to 1 month. Do not heat the kit over 37 °C or use uncontrollable sources of heat (e.g. microwave appliances).
Do not refreeze the kit. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage.
Overtrypsination causes irreversible damage.
Because of the tryptic activity of this passage kit, do not exceed the recommended incubation period. Exposing the cells too long will cause irreversible damages to your culture.

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