Hľadali ste: Činidla pro nukleové kyseliny

Dodávateľ: ENZO LIFE SCIENCES

Opis: Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.

New Product

Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Forensic DNA Kit is designed to provide a rapid and easy method for the isolation of genomic DNA from forensic samples such as dry blood, buccal swabs, and semen samples for consistent PCR and Southern analysis. This kit can also be used for the preparation of genomic DNA from mouse tail snips, whole blood, buffy coat, serum, and plasma.

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Dodávateľ: OMEGA BIOTEK

Opis: Souprava E.Z.N.A.® SP Plant DNA Kit je navržena speciálně pro rychlou a spolehlivou izolaci vysoce kvalitní celkové buněčné DNA ze širokého spektra rostlinných druhů. Touto metodou je možno do 40 minut zpracovat až 100 mg čerstvého, případně až 30 mg suchého vzorku. Takto purifikovaná DNA je vhodná pro následné aplikace, včetně PCR, restrikčního štěpení a hybridizačních technik.

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Dodávateľ: OMEGA BIOTEK

Opis: Plazmid izolovaný pomocí tradičních purifikačních metod běžně obsahuje vysoké hladiny endotoxinů, které mohou významně interferovat s následnými transfekčními experimenty. U souprav E.Z.N.A.® Endo-Free Plasmid Kit je do postupu plazmidové purifikace za účelem získání plazmidu o vysoké kvalitě vhodného pro transfekci včleněn krok efektivního odstranění endotoxinů.

KBÚ – slovenskú verziu si vyžiadajte na regulatory.cz@avatorsciences.com Certifikáty

Dodávateľ: OMEGA BIOTEK

Opis: The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

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Dodávateľ: Thermo Fisher Scientific

Opis: T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

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Dodávateľ: Thermo Fisher Scientific

Opis: AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Dodávateľ: Thermo Fisher Scientific

Opis: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Certifikáty

Dodávateľ: Quantabio

Opis: AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.

Certifikáty

Dodávateľ: TriLink BioTechnologies

Opis: TriLink® N1-Propylpseudouridine-5'-Triphosphate

New Product

Dodávateľ: TriLink BioTechnologies

Opis: 2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

New Product

Dodávateľ: TriLink BioTechnologies

Opis: TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

New Product

Dodávateľ: Corning

Opis: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Katalógové číslo: (MAG-PCR-NM-5)

Dodávateľ: Corning

Opis: The AxyPrep™ Mag PCR Normalizer Kit utilizes a paramagnetic bead-based purification system for PCR clean up.

Merná jednotka: 1 * 1 KIT

Predaj

Dodávateľ: Corning

Opis: Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.

Dodávateľ: Quantabio

Opis: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

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